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 Molecular Physiology

 Methods

In our group, we employ electrophysiological, molecular biological and imaging approaches to study mechanisms of learning and memory at single synapses and neuronal networks.

In particular we use single particle tracking, FRAP and PALM to study the dynamic of surface molecules.

Electrophysiological methods like patch-clamp and extracellular field recordings are used in combination with the imaging approaches.

Single molecule tracking of membrane lipids

Glycolyl-phosphatidylinostiol (GPI) coupled GFP labeled with Quantum dot Dendritic compartment Axonal compartment

Fig.: Example of a quantum dot labeled GFP-GPI on dendrites and axons of cultured hippocampal neurons 14 DIV, trajectories (red) are from 1 min recording at 30 Hz acquisition frequency